Characterization of Protein-Protein Interactions via Static and Dynamic Light Scattering
نویسندگان
چکیده
Light scattering in its various flavors constitutes a label-free, non-destructive probe of macromolecular interactions in solution, providing a direct indication of the formation or dissociation of complexes by measuring changes in the average molar mass or molecular radius as a function of solution composition and time. It is a first-principles technique, thoroughly grounded in thermodynamics, permitting quantitative analysis of key properties such as stoichiometry, equilibrium association constants, and reaction rate parameters.
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